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[(o)-5.6(r)-5.5(der)-306.6(t)3.7(o)-330.8(t)3.7(a)-5.6(k)22.2(e)-302.1(i)1(n)25.9(t)3.7(o)-330.8(a)-5.6(cco)-5.6(un)25.9(t)-297.4(t)3.7(he)-326.1(kinetic)-326.1(p)1.8(r)-5.5(o)-5.6(p)-22.3(e)-0.9(r)-5.5(t)3.7(ies)-315.9(o)-5.6(f)-308.4(m)2.5(a)-5.6(n)25.9(y)-315.1(e)-0.9(nzymes,)-312.1(w)23.9(e)-314.1(a)-5.6(sso)-29.7(cia)-5.6(t)3.7(e)]TJ /F9 1 Tf 0 -1.1925 TD 0.0042 Tc [(r)52.5(a)9.4(te)-439.2(c)54.7(o)9.4(nsta)9.4(nts)]TJ /F2 1 Tf 6.673 0 TD 0.0005 Tc [(with)-443.7(eac)23.4(h)-419.6(reaction.)-420.3(T)0(h)26.1(u)2(s)-436.2(t)3.9(he)-434.3(enzyme)]TJ /F8 1 Tf 17.092 0 TD 0 Tc (E)Tj /F2 1 Tf 1.2286 0 TD -0.0012 Tc [(ca)-7.1(n)-433.3(c)-2.4(o)-7.1(m)25.1(bine)-424(with)-445.4(the)]TJ -24.9936 -1.2045 TD -0.0006 Tc [(substr)-6.4(a)-6.5(t)2.8(e)]TJ /F8 1 Tf 4.3603 0 TD 0 Tc (A)Tj /F2 1 Tf 1.0961 0 TD -0.0034 Tc [(to)-346.6(fo)-9.3(r)-9.2(m)-350.5(the)]TJ /F8 1 Tf 5.348 0 TD 0 Tc (A)Tj /F11 1 Tf 0.7468 0 TD (|)Tj /F8 1 Tf 0.277 0 TD (E)Tj /F2 1 Tf 1.1443 0 TD 0.0049 Tc [(com)7.1(p)6.4(l)5.6(e)3.7(x)-334.6(w)4.4(i)5.6(t)8.3(h)-342.9(r)-0.9(at)8.3(e)-333.6(c)3.7(on)6.4(st)8.3(an)30.5(t)]TJ /F8 1 Tf 12.3342 0 TD 0 Tc (k)Tj /F3 1 Tf 6.9738 0 0 6.9738 392.04 513.9 Tm (1)Tj /F2 1 Tf 9.9626 0 0 9.9626 396.48 515.34 Tm 0.3486 Tc [(.T)348.1(h)350.1(e)]TJ /F8 1 Tf 2.6861 0 TD 0 Tc (A)Tj /F11 1 Tf 0.7468 0 TD (|)Tj /F8 1 Tf 0.277 0 TD (E)Tj /F2 1 Tf 1.1443 0 TD 0.0007 Tc (complex)Tj -31.1243 -1.1925 TD 0.0025 Tc [(can)-393.5(d)4(isso)-27.5(ciate)-396.2(t)5.9(o)-400.9(E)-413(an)]TJ 9.9613 0 TD -0.0018 Tc [(d)-397.8(A)-408.1(with)-409.9(r)-7.6(a)-7.7(te)-400.5(co)-7.7(ns)-4.8(ta)-7.7(n)23.8(t)]TJ /F8 1 Tf 10.6961 0 TD 0 Tc (k)Tj /F3 1 Tf 6.9738 0 0 6.9738 345.72 502.02 Tm (2)Tj /F2 1 Tf 9.9626 0 0 9.9626 350.16 503.46 Tm -0.0013 Tc [(,)-410.1(o)-7.2(r)-392.6(f)3.1(o)-7.2(r)-7.1(m)-408.6(t)2.1(he)-412.1(pr)-7.1(o)-31.3(duct)-395.3(B)-401.1(with)]TJ -21.6208 -1.2045 TD 0.0059 Tc [(rat)9.3(e)]TJ /F8 1 Tf 2.0597 0 TD 0 Tc (k)Tj /F3 1 Tf 6.9738 0 0 6.9738 160.44 490.02 Tm (3)Tj /F2 1 Tf 9.9626 0 0 9.9626 164.88 491.46 Tm (:)Tj /F8 1 Tf 8.4435 -3.1558 TD (A)Tj /F2 1 Tf 0.9757 0 TD (+)Tj /F8 1 Tf 0.9997 0 TD (E)Tj /F10 1 Tf 6.9738 0 0 6.9738 280.56 472.62 Tm (k)Tj /F14 1 Tf 4.9813 0 0 4.9813 284.76 471.66 Tm (1)Tj /F11 1 Tf 9.9626 0 0 9.9626 278.04 466.86 Tm ()Tj 0.4457 0 TD ()Tj -0.4457 -1.1925 TD ()Tj 0.6745 0 TD ()Tj /F10 1 Tf 6.9738 0 0 6.9738 281.88 448.5 Tm (k)Tj /F14 1 Tf 4.9813 0 0 4.9813 286.08 447.54 Tm (2)Tj /F8 1 Tf 9.9626 0 0 9.9626 293.88 460.02 Tm (A)Tj /F11 1 Tf 0.7468 0 TD (|)Tj /F8 1 Tf 0.277 0 TD (E)Tj /F10 1 Tf 6.9738 0 0 6.9738 317.28 465.78 Tm (k)Tj /F14 1 Tf 4.9813 0 0 4.9813 321.48 464.82 Tm (3)Tj /F11 1 Tf 9.9626 0 0 9.9626 314.88 460.02 Tm ()Tj 0.4457 0 TD ()Tj /F8 1 Tf 1.2768 0 TD (B)Tj /F2 1 Tf 1.0359 0 TD (+)Tj /F8 1 Tf 0.9997 0 TD 0.394 Tc (E.)Tj /F2 1 Tf 9.9251 0 TD -0.0028 Tc [(\(MA1)-8.7(\))]TJ -30.2571 -2.7463 TD -0.0003 Tc [(This)-497.2(simple)-495.4(ma)-6.2(ss-a)-6.2(c)-1.5(tio)-6.2(n)-480.6(mo)-30.3(del)-505.4(i)0.4(s)-497.2(r)-6.1(ela)-6.2(t)3.1(ed)-492.7(to)-500(the)-507.4(M)0.9(ic)22.6(ha)-6.2(elis-Men)25.3(ten)-480.6(E)-6.3(qua)-6.2(-)]TJ -1.5056 -1.2045 TD [(tio)-6.3(n)-336.2(MM)-336.4(a)-6.3(s)-328.7(descr)-6.2(i)0.3(b)-23(e)-1.6(d)-324.1(p)1.1(r)-6.2(e)-1.6(vio)-6.3(u)1.1(sly)-315.8(b)25.2(y)-339.9(t)3(he)-338.9(fo)-6.3(llo)17.8(wing)-319.5(co)-6.3(nstr)-6.2(a)-6.3(i)0.3(n)25.2(t)3(s:)]TJ /F8 1 Tf 14.2372 -1.9874 TD 0 Tc (k)Tj /F3 1 Tf 6.9738 0 0 6.9738 281.76 399.42 Tm (2)Tj /F2 1 Tf 9.9626 0 0 9.9626 288.48 400.86 Tm (+)Tj /F8 1 Tf 0.9997 0 TD (k)Tj /F3 1 Tf 6.9738 0 0 6.9738 303.6 399.42 Tm (3)Tj ET q 31.56 0 0 -0.48 276.588 396.888 cm /Im1 Do Q BT /F8 1 Tf 9.9626 0 0 9.9626 287.52 387.3 Tm (k)Tj /F3 1 Tf 6.9738 0 0 6.9738 292.68 385.86 Tm (1)Tj /F2 1 Tf 9.9626 0 0 9.9626 312.12 394.14 Tm (=)Tj /F8 1 Tf 1.0479 0 TD (K)Tj /F10 1 Tf 6.9738 0 0 6.9738 331.08 392.7 Tm (M)Tj /F2 1 Tf 9.9626 0 0 9.9626 467.88 394.14 Tm -0.0034 Tc [(\(4)-9.3(\))]TJ -31.9313 -2.5054 TD 0.0004 Tc [(wher)-5.4(e)]TJ /F8 1 Tf 2.8908 0 TD 0 Tc (k)Tj /F3 1 Tf 6.9738 0 0 6.9738 183.72 367.74 Tm (3)Tj /F2 1 Tf 9.9626 0 0 9.9626 190.92 369.18 Tm (=)Tj /F8 1 Tf 1.06 0 TD (k)Tj /F2 1 Tf 0.5179 -0.277 TD 0.0036 Tc (cat)Tj 1.6622 0.277 TD 0 Tc (=)Tj /F10 1 Tf 6.9738 0 0 6.9738 234.96 374.1 Tm (V)Tj /F2 1 Tf 9.9626 0 0 9.9626 239.64 372.18 Tm -0.0022 Tc [(ma)-8.1(x)]TJ ET q 23.76 0 0 -0.48 234.948 371.928 cm /Im1 Do Q BT /F3 1 Tf 6.9738 0 0 6.9738 238.8 365.7 Tm 0 Tc ([)Tj /F10 1 Tf 0.327 0 TD (E)Tj /F15 1 Tf 4.9813 0 0 4.9813 246.96 364.74 Tm (T)Tj /F3 1 Tf 6.9738 0 0 6.9738 252.48 365.7 Tm (])Tj /F2 1 Tf 9.9626 0 0 9.9626 263.16 369.18 Tm 0.0055 Tc [(as)-322.8(i)6.2(n)-330.3(Equ)7(a)-0.4(t)8.9(i)6.2(on)-306.2(2.)]TJ -11.3825 -1.3129 TD 0.0015 Tc [(W)89.8(e)-337(can)-334.3(d)3(eriv)23.4(e)-324.9(a)-341.7(set)-344.4(o)-4.4(f)-331.3(d)3(i)6.7(eren)27.1(tial)-335(equa)]TJ 17.1282 0 TD 0.0041 Tc [(t)7.5(i)4.8(on)5.6(s,)-320.4(see)-334.4(E)-1.9(qu)5.6(at)7.5(i)4.8(o)-1.8(n)-331.7(5)-327(f)8.5(r)-1.7(om)-330.9(t)7.5(h)5.6(e)-346.4(m)6.3(ass-)]TJ -18.6338 -1.2045 TD 0.0043 Tc [(act)7.7(i)5(on)-319.4(d)5.8(e)3.1(scri)5(p)5.8(t)7.7(i)5(o)-1.6(n)-319.4(g)-1.6(i)5(v)26.2(en)-319.4(i)5(n)-331.5(Equ)5.8(a)-1.6(t)7.7(i)5(on)-307.4(M)5.5(A)7.5(1:)]TJ /F10 1 Tf 6.9738 0 0 6.9738 207.84 325.86 Tm 0 Tc (d)Tj /F3 1 Tf 0.6023 0 TD ([)Tj /F10 1 Tf 0.327 0 TD (A)Tj /F3 1 Tf 0.8604 0 TD (])Tj ET q 14.76 0 0 -0.48 207.828 323.808 cm /Im1 Do Q BT /F10 1 Tf 6.9738 0 0 6.9738 211.56 317.7 Tm 0.0076 Tc (dt)Tj /F2 1 Tf 9.9626 0 0 9.9626 226.44 321.06 Tm 0 Tc (=)Tj /F11 1 Tf 1.06 0 TD ()Tj /F8 1 Tf 0.7829 0 TD (k)Tj /F3 1 Tf 6.9738 0 0 6.9738 249.96 319.62 Tm (1)Tj /F11 1 Tf 9.9626 0 0 9.9626 256.56 321.06 Tm ()Tj /F2 1 Tf 0.9997 0 TD ([)Tj /F8 1 Tf 0.277 0 TD (A)Tj /F2 1 Tf 0.7468 0 TD (])Tj /F11 1 Tf 0.5059 0 TD ()Tj /F2 1 Tf 0.9997 0 TD ([)Tj /F8 1 Tf 0.277 0 TD (E)Tj /F2 1 Tf 0.795 0 TD 0.2883 Tc (]+)Tj /F8 1 Tf 1.3491 0 TD 0 Tc (k)Tj /F3 1 Tf 6.9738 0 0 6.9738 321 319.62 Tm (2)Tj /F11 1 Tf 9.9626 0 0 9.9626 327.6 321.06 Tm ()Tj /F2 1 Tf 0.9997 0 TD ([)Tj /F8 1 Tf 0.277 0 TD (A)Tj /F11 1 Tf 0.7468 0 TD (|)Tj /F8 1 Tf 0.277 0 TD (E)Tj /F2 1 Tf 0.795 0 TD (])Tj /F10 1 Tf 6.9738 0 0 6.9738 199.2 300.3 Tm (d)Tj /F3 1 Tf 0.6023 0 TD ([)Tj /F10 1 Tf 0.327 0 TD (A)Tj /F16 1 Tf 0.8604 0 TD (|)Tj /F10 1 Tf 0.3442 0 TD (E)Tj /F3 1 Tf 0.8948 0 TD (])Tj ET q 23.28 0 0 -0.48 199.188 298.248 cm /Im1 Do Q BT /F10 1 Tf 6.9738 0 0 6.9738 207.24 292.02 Tm 0.0076 Tc (dt)Tj /F2 1 Tf 9.9626 0 0 9.9626 226.44 295.5 Tm 0 Tc (=)Tj /F8 1 Tf 1.8309 0 TD (k)Tj /F3 1 Tf 6.9738 0 0 6.9738 249.84 294.06 Tm (1)Tj /F11 1 Tf 9.9626 0 0 9.9626 256.56 295.5 Tm ()Tj /F2 1 Tf 0.9997 0 TD ([)Tj /F8 1 Tf 0.277 0 TD (A)Tj /F2 1 Tf 0.7468 0 TD (])Tj /F11 1 Tf 0.5059 0 TD ()Tj /F2 1 Tf 0.9997 0 TD ([)Tj /F8 1 Tf 0.277 0 TD (E)Tj /F2 1 Tf 0.795 0 TD (])Tj /F11 1 Tf 0.5661 0 TD ()Tj /F8 1 Tf 0.7829 0 TD (k)Tj /F3 1 Tf 6.9738 0 0 6.9738 321 294.06 Tm (2)Tj /F11 1 Tf 9.9626 0 0 9.9626 327.6 295.5 Tm ()Tj /F2 1 Tf 0.9997 0 TD ([)Tj /F8 1 Tf 0.277 0 TD (A)Tj /F11 1 Tf 0.7468 0 TD (|)Tj /F8 1 Tf 0.277 0 TD (E)Tj /F2 1 Tf 0.795 0 TD (])Tj /F11 1 Tf 0.5661 0 TD ()Tj /F8 1 Tf 0.7829 0 TD (k)Tj /F3 1 Tf 6.9738 0 0 6.9738 377.04 294.06 Tm (3)Tj /F11 1 Tf 9.9626 0 0 9.9626 383.64 295.5 Tm ()Tj /F2 1 Tf 0.9997 0 TD ([)Tj /F8 1 Tf 0.277 0 TD (A)Tj /F11 1 Tf 0.7468 0 TD (|)Tj /F8 1 Tf 0.277 0 TD (E)Tj /F2 1 Tf 0.795 0 TD (])Tj /F10 1 Tf 6.9738 0 0 6.9738 207.48 274.74 Tm (d)Tj /F3 1 Tf 0.6023 0 TD ([)Tj /F10 1 Tf 0.327 0 TD (B)Tj /F3 1 Tf 0.9121 0 TD (])Tj ET q 15.12 0 0 -0.48 207.468 272.688 cm /Im1 Do Q BT /F10 1 Tf 6.9738 0 0 6.9738 211.32 266.46 Tm 0.0076 Tc (dt)Tj /F2 1 Tf 9.9626 0 0 9.9626 226.44 269.82 Tm 13.0378 Tc (=+)Tj /F8 1 Tf 14.5986 0 TD 0 Tc (k)Tj /F3 1 Tf 6.9738 0 0 6.9738 377.04 268.38 Tm (3)Tj /F11 1 Tf 9.9626 0 0 9.9626 383.64 269.82 Tm ()Tj /F2 1 Tf 0.9997 0 TD ([)Tj /F8 1 Tf 0.277 0 TD (A)Tj /F11 1 Tf 0.7468 0 TD (|)Tj /F8 1 Tf 0.277 0 TD (E)Tj /F2 1 Tf 0.795 0 TD (])Tj /F10 1 Tf 6.9738 0 0 6.9738 207.6 249.06 Tm (d)Tj /F3 1 Tf 0.6023 0 TD ([)Tj /F10 1 Tf 0.327 0 TD (E)Tj /F3 1 Tf 0.8948 0 TD (])Tj ET q 15 0 0 -0.48 207.588 247.008 cm /Im1 Do Q BT /F10 1 Tf 6.9738 0 0 6.9738 211.44 240.78 Tm 0.0076 Tc (dt)Tj /F2 1 Tf 9.9626 0 0 9.9626 226.44 244.26 Tm 0 Tc (=)Tj /F11 1 Tf 1.06 0 TD ()Tj /F8 1 Tf 0.7829 0 TD (k)Tj /F3 1 Tf 6.9738 0 0 6.9738 249.96 242.82 Tm (1)Tj /F11 1 Tf 9.9626 0 0 9.9626 256.56 244.26 Tm ()Tj /F2 1 Tf 0.9997 0 TD ([)Tj /F8 1 Tf 0.277 0 TD (A)Tj /F2 1 Tf 0.7468 0 TD (])Tj /F11 1 Tf 0.5059 0 TD ()Tj /F2 1 Tf 0.9997 0 TD ([)Tj /F8 1 Tf 0.277 0 TD (E)Tj /F2 1 Tf 0.795 0 TD 0.2883 Tc (]+)Tj /F8 1 Tf 1.3491 0 TD 0 Tc (k)Tj /F3 1 Tf 6.9738 0 0 6.9738 321 242.82 Tm (2)Tj /F11 1 Tf 9.9626 0 0 9.9626 327.6 244.26 Tm ()Tj /F2 1 Tf 0.9997 0 TD ([)Tj /F8 1 Tf 0.277 0 TD (A)Tj /F11 1 Tf 0.7468 0 TD (|)Tj /F8 1 Tf 0.277 0 TD (E)Tj /F2 1 Tf 0.795 0 TD 0.2883 Tc (]+)Tj 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