SUMMARY OF THE COMMAND LINE USAGE Clustal W is designed to be run interactively. However, there are many situations where it is convenient to run it from the command line, especially if you wish to run it from another piece of software (e.g. SeqApp or GDE). All parameters can be set from the command line by giving options after the clustalw command. On UNIX options should be preceded by '-'. If anything is put on the command line, the program will (attempt to) carry out whatever is requested and will exit. If you wish to use the command line to set some parameters and then go into interactive mode, use the command line switch: interactive .... e.g. clustalw -quicktree -interactive on UNIX will set the default initial alignment mode to fast/approximate and will then go to the main menu. To see a list of all the command line parameters, type: clustalw -options on UNIX and you will see a list with no explanation. To get (VERY BRIEF) help on command line usage, use the /HELP or /CHECK (-help or -check on UNIX systems) options.