LOCUS       ECOICD                  1568 bp    DNA     linear   BCT 26-APR-1993
DEFINITION  E.coli icd gene encoding isocitrate dehydrogenase, complete cds.
ACCESSION   J02799
VERSION     J02799.1  GI:146431
KEYWORDS    icd gene; isocitrate dehydrogenase.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Thorsness,P.E. and Koshland,D.E.Jr.
  JOURNAL   Unpublished (1987)
REFERENCE   2  (bases 291 to 1538)
  AUTHORS   Thorsness,P.E. and Koshland,D.E. Jr.
  TITLE     Inactivation of isocitrate dehydrogenase by phosphorylation is
            mediated by the negative charge of the phosphate
  JOURNAL   J. Biol. Chem. 262 (22), 10422-10425 (1987)
   PUBMED   3112144
COMMENT     Original source text: E.coli DNA, clone pTK512.
            Draft entry and printed copy of sequence [2],[1] kindly provided by
            P.E.Thorsness, 01-JUL-1987.
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:562"
     CDS             291..1541
                     /note="isocitrate dehydrogenase (icd; EC 1.1.1.42)"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="AAA24006.1"
                     /db_xref="GI:146432"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMDGAKLLKCSEFGDAIIENM"
ORIGIN      MluI site; 25.3 min on K12 map.
        1 cgcgtggcgt ggttttcagg tttacgcctg gtagaacgtt gcgagctgaa tcgcttaacc
       61 tggtgatttc taaaagaagt tttttgcatg gtattttcag agattatgaa ttgccgcatt
      121 atagcctaat aacgcgcatc tttcatgacg gcaaacaata gggtagtatt gacaagccaa
      181 ttacaaatca ttaacaaaaa attgctctaa agcatccgta tcgcaggacg caaacgcata
      241 tgcaacgtgg tggcagacga gcaaaccagt agcgctcgaa ggagaggtga atggaaagta
      301 aagtagttgt tccggcacaa ggcaagaaga tcaccctgca aaacggcaaa ctcaacgttc
      361 ctgaaaatcc gattatccct tacattgaag gtgatggaat cggtgtagat gtaaccccag
      421 ccatgctgaa agtggtcgac gctgcagtcg agaaagccta taaaggcgag cgtaaaatct
      481 cctggatgga aatttacacc ggtgaaaaat ccacacaggt ttatggtcag gacgtctggc
      541 tgcctgctga aactcttgat ctgattcgtg aatatcgcgt tgccattaaa ggtccgctga
      601 ccactccggt tggtggcggt attcgctctc tgaacgttgc cctgcgccag gaactggatc
      661 tctacatctg cctgcgtccg gtacgttact atcagggcac tccaagcccg gttaaacacc
      721 ctgaactgac cgatatggtt atcttccgtg aaaactcgga agacatttat gcgggtatcg
      781 aatggaaagc agactctgcc gacgccgaga aagtgattaa attcctgcgt gaagagatgg
      841 gggtgaagaa aattcgcttc ccggaacatt gtggtatcgg tattaagccg tgttcggaag
      901 aaggcaccaa acgtctggtt cgtgcagcga tcgaatacgc aattgctaac gatcgtgact
      961 ctgtgactct ggtgcacaaa ggcaacatca tgaagttcac cgaaggagcg tttaaagact
     1021 ggggctacca gctggcgcgt gaagagtttg gcggtgaact gatcgacggt ggcccgtggc
     1081 tgaaagttaa aaacccgaac actggcaaag agatcgtcat taaagacgtg attgctgatg
     1141 cattcctgca acagatcctg ctgcgtccgg ctgaatatga tgttatcgcc tgtatgaacc
     1201 tgaacggtga ctacatttct gacgccctgg cagcgcaggt tggcggtatc ggtatcgccc
     1261 ctggtgcaaa catcggtgac gaatgcgccc tgtttgaagc cacccacggt actgcgccga
     1321 aatatgccgg tcaggacaaa gtaaatcctg gctctattat tctctccgct gagatgatgc
     1381 tgcgccacat gggttggacc gaagcggctg acttaattgt taaaggtatg gaaggcgcaa
     1441 tcaacgcgaa aaccgtaacc tatgacttcg agcgtctgat ggatggcgct aaactgctga
     1501 aatgttcaga gtttggtgac gcgatcatcg aaaacatgta atgccgtagt ttgttaaatt
     1561 tattaacg
//